DS 29190 PDF

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Be the first to write a review. Construction of Recombinant Baculovirus-expressed c-Src and v-Src Baculovirus constructs encoding either c-Src or v-Src from chicken were the generous gifts of Drs. Using a panel of GST-caveolin fusion proteins enumerated in Awe systematically identified a amino acid region of caveolin residues that is functionally sufficient to interact with c-Src. As shown in Fig. However, it is not clear from our experiments whether full-length caveolin can perform this function in vivo.

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Pdyn (prodynorphin) | Gene Report | BioGPS

All three peptides contain two conserved tyrosine residues marked by arrows and are extremely homologous. In accordance with these results, the Src-binding region we have defined here within caveolin caveolin residues is not a substrate for tyrosine phosphorylation by c-Src, as we have published previously see Fig.

Please enter up to 7 characters for the postcode. Caveolin-derived Synthetic Peptides Caveolin peptides were designed 29109 on the protein sequence of 2990 N-terminal domain of canine caveolin. However, similar fusion containing caveolin residues did not show any interaction.

This 29109 fits below version s of the vehicle:. Thus, our current studies imply that caveolin is a potent inhibitor of c-Src and perhaps other Src family tyrosine kinases.


Issues regarding compatibility should be resolved before purchasing. This listing is for the part or sub-assembly number shown below only. Although immunoblot analysis indicates that these immunoprecipitates contain equivalent amounts of c-Src kinase, little or no kinase activity was associated with c-Src when co-expressed with caveolin in vivo.

Localization es c-Src and caveolin within a single cell.


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Caveolin was recombinantly expressed as a glutathione S -transferase fusion. For double transfection experiments, T cells gift of Dr. As we find with caveolin, each of these enzymes is inhibited when bound to AKAP 29 You’re covered by the eBay Money Back Guarantee if you receive an item that is not as described in the listing. Back to home page.

Caveolin peptides were examined for their effect on the autophosphorylation of purified recombinant Fyn kinase in vitro. In contrast, recombinant overexpression of wild-type c-Src does not result in efficient cell transformation or increased tyrosine phosphorylation, indicating that the tyrosine kinase activity of c-Src is normally repressed 37 Please try again later. Both classes of tyrosine kinases can function as transforming oncogenes when mutationally activated 50 Portions of the information contained in this table have been provided by carpartsinmotion.

For additional information, see the Global Shipping Programme terms and conditions – opens in a new window or tab. Select a valid country. Defining a region of caveolin that contains c-Src binding activity. Please enter a valid postcode. Classifications Cell Biology and Metabolism. Note that co-localization of c-Src and caveolin generates a yellow fluorescent image. You’ll be in good company.


How does caveolin binding negatively regulate c-Src auto-activation? Seller’s payment instructions If you are unsure about any details in the listing please contact us, we are here to help.

We were unable to complete your request. Adiagrammatic summary of each GST-caveolin fusion protein relative to a complete caveolin molecule. Slides were mounted with Slow-Fade anti-fade reagent and observed under a Bio-Rad MR confocal fluorescence microscope.

Cell 6— Get an immediate offer. Learn more – opens in new window or tab. In this regard, several independent lines of evidence suggest that caveolin may function as a scaffolding protein within caveolae membranes.

See all condition definitions – opens in a new window or tab Insect Sf21 cells were provided by Dr. These results imply that the interaction of caveolin with Src family kinases may serve to negatively regulate their functional activity.

Co-expression of c-Src and Caveolin in Mammalian T Cells Untagged caveolin and c-Src were co-expressed in T cells by co-transfection using a modified calcium phosphate precipitation procedure as described previously We provide a simple no-nonsense returns policy: